Rationally designed imidazole derivative as colorimetric and fluorometric sensor for selective, qualitative and quantitative cyanide ion detection in real time samples.

Abstract

A new imidazole derivative of 1,2-diaminoanthraquinone and fluorene-2-carboxaldehyde was designed as a sensor B2 to selectively detect the cyanide (CN-) ion through colorimetric and/or fluorometric methods. The photochemical characterizations of sensor B2 were tested using absorption and emission spectral studies in CH3CN-H2O (8:2) semi-aqueous medium. An excited state proton transfer process (ESIPT) was proved by theoretical and spectral studies. The colorimetric and fluorescence detection limit of CN- ion was found to be 5.3×10-6M and 4.11×10-8M, respectively. 1H NMR titration, electrochemical and DFT studies were supported the removal of -NH proton from B2. In order to utilize this sensor in real-time applications, we developed a test cassette which is coated with sensor B2 detected the presence of CN- ion in the food sample with endogenous cyanide ion.