Abstract
A sensing platform with both ratiometric fluorescence and colorimetric responses towards copper(II) ions (Cu2+) andD-penicillamine (D-pen) was constructed based on carbon dots (CDs). o-Phenylenediamine (OPD) was employed as a chromogenic development reagent for reaction with Cu2+to generate the oxidation product 2,3-diaminophenazine (oxOPD), which not only emits green fluorescence at 555nm, but also quenches the blue fluorescence of CDs at 443nm via the inner filter effect (IFE) and Förster resonance energy transfer (FRET). Additionally, oxOPD exhibits obvious absorption at 420nm. Since the intense chelation affinity of D-pen to Cu2+ greatly inhibits the oxidation of OPD, the intensity ratio of fluorescence at 443nm to that at 555nm (F443/F555) and the absorbance at 420nm (A420) were conveniently employed as spectral response signals to represent the amount of D-pen introduced into the testing system. This dual-signal sensing platform exhibits excellent selectivity and sensitivity towards both Cu2+ and D-pen, with low detection limits of 0.019μM and 0.092μM, respectively. In addition, the low cytotoxicity of the testing reagents involved in the proposed sensing platform facilitates its application for live cell imaging.
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