Abstract
Abstract The small signal transmitters SO2 can elaborately modulate signal transduction pathways. Endogenous formaldehyde (FA) can toxify protein and DNA as a crosslinking agent besides functions in the aspect of spatial memory and cognitive ability. Both SO2 and FA are tightly associated with neurological disorders, cardiovascular diseases. Therefore, it is significant and challenging to monitor the real-time dynamic fluctuation of SO2 and FA in cells or animals. Toward this end, for the first time, a near-infrared (NIR) dual-emission fluorescent probe (BCou-BP) for SO2 and FA was developed based on the rational combination of benzypylium and benzo[g]coumarin cores through tuning the conjugation and rigidity of the fluorophore units. Thus, this rational design affords the new cationic probe the ratiometric detection ability (F604/F694) toward mitochondrial SO2 with a fast response time of 10 s, as well as toward FA in 170 s. Endogenous FA could restore the fluorescence at 694 nm of BCou-BP due to the capture of SO2 from decomposition of the adduct BCou-BP-SO3H. The powerful probe succeeded in real-time visualizing the dynamic change of endogenous mitochondrial SO2 and FA in living cells and mice.
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