Rates of Mortality, Growth, and Biomass Production of Lymnaea palustris During Chronic Exposure to Lead

Abstract

Lymnaea palustris, a freshwater snail, was exposed to lead in a continuous-flow system from newly laid eggs until reproductive maturity. Lead, at concentrations as low as 19 μg∙L−1, caused a significant increase in the mortality rate, although the growth rate was not affected. The lead-induced mortality was proportional to the lead concentration raised to the exponent 2.5. The analysis of mortality as a rate function was found superior to the use of the more conventional LC50 (concentration resulting in 50% mortality). A plot of the logarithm of the lead-induced mortality against log lead concentration is linear and has the same slope as plots of double logarithms of the inverse survival at any one time versus log lead concentration or log time versus log LC50 or log LT50 (time to 50% mortality) versus log lead concentration. The mortality rate, unlike the percent mortality, is independent of time and can be combined directly with the growth rate to estimate the biomass production rate. A 50% drop in the rate of biomass production was observed at 36 μg∙L−1 and a drop to 0 production at 48 μg∙L−1. Lead uptake in whole snails was found to be proportional to the lead concentration in the water, giving a concentration factor of 8500 on a dry weight basis. Key words: snails, lead, toxicity, biomass production, bioaccumulation