Rat Model of Cecal Ligation and Puncture versus Colon Ascendens Stent Peritonitis: Comparative Study for Oxidative Stress

Lei Chen,Daiyin Cao,Enhe Liu,Chaoxing Xiao,Qiuye Kou,Mengran Xiong

doi:10.4236/aid.2017.73009

Abstract

The CLP and CASP model as important research tools have been widely utilized in septic OS. In this study, we compare the oxidative stress condition of CLP and CASP rat models. Compared with sham operation group, infection condition and oxidative stress index were significantly different in CLP and CASP groups. Compared with CLP group, there was no significant difference in natural death rate, bacterial colony positive rate, infection condition, center venous oxygen saturation, and serum levels of TNF-α in the CASP group; whereas, MDA decreased significantly at 72 hours, T-SOD increased considerably at 12 and 72 hours after operation in the CASP group. The serum NO significantly decreased at all time-points except at 12 hours post-operation. Together, our study demonstrated the similarity of oxidative stress condition between CLP and CASP models. However, CASP rats had higher serum NO level than CLP rats.

Highlights

Sepsis refers to the systemic inflammatory response syndrome (SIRS) caused by infections [1], which is the common pathophysiological process of multiple diseases as a consequence of autoimmune injury mediated by large amount cytokines and inflammatory mediators [2]
There was a significant difference in survival rate between the sham operation and cecal ligation and puncture (CLP) or colon ascendens stent peritonitis (CASP) group (P < 0.05), there was no significant difference between the CLP and CASP groups (P > 0.05) (Figure 1)
The oxidative stress condition was compared in the two models, and it was found that the oxidative stress parameters such as MDA and NO were significantly increased in both CLP and CASP models 12 hours after operation compared with the sham operation group (P < 0.05)

Summary

Introduction

Sepsis refers to the systemic inflammatory response syndrome (SIRS) caused by infections [1], which is the common pathophysiological process of multiple diseases as a consequence of autoimmune injury mediated by large amount cytokines and inflammatory mediators [2]. Further development of sepsis results in severe conditions including septic shock, severe sepsis, multiple organ dysfunction syndromes (MODS), and even death. The pathogenesis mechanism of sepsis is very complicated; the major pathophysiological processes include cytokine storm, inflammatory waterfall-like reaction, enteral bacterial translocation, intestinal-derived endotoxin, the interplay between coagulation and inflammation systems, oxidative stress (OS), immune cell apoptosis, microcirculation and mitochondrial dysfunction [10] [11] [12] [13]. Maier et al [15] have suggested that CASP closely mimics the clinical course of diffuse peritonitis, whereas CLP more closely resembles intra-abdominal abscess formation

Methods

Results

Conclusion