Rat bone marrow stromal cells-seeded porous gelatin/tricalcium phosphate/oligomeric proanthocyanidins composite scaffold for bone repair

Abstract

Repair of bone defects remains a major challenge in orthopaedic surgery. Bone tissue engineering is an attractive approach for treating bone loss in various shapes and amounts. The aim of this study was to prepare and evaluate the feasibility of a porous scaffold, which was composed of oligomeric proanthocyanidin crosslinked gelatin mixed with β-tricalcium phosphate (GTP) and was seeded with bone marrow stromal cells (BMSCs) as a bone substitute. GTP scaffolds were made porous using a salt-leaching method. The physicochemical properties of the scaffold were evaluated to determine the optimal salt:composite weight ratio. The results indicated that the GTP scaffold had a favourable macroporous structure and higher porosity when the salt:composite weight ratio was 4:1. Cytotoxic tests demonstrated that extracts from the GTP scaffolds promoted the proliferation of BMSCs. Rat BMSCs were seeded on a GTP scaffold and cultured in a spinner flask. After 2 weeks of culture, scanning electron microscopy observation showed that the cells adhered well to the surfaces of the pores in the scaffold. Moreover, this study explored the biological response of rat calvarial bone to the scaffold to evaluate its potential in bone tissue engineering. Bone defects were filled with BMSC-seeded GTP scaffold and acellular GTP scaffold. After 8 weeks, the scaffold induced new bone formation at a bone defect, as was confirmed by X-ray microradiography and histology. The BMSC-seeded scaffold induced more new bone formation than did an acellular scaffold. These observations suggest that the BMSCs-seeded GTP scaffold can promote the regeneration of defective bone tissue.