Abstract
MRSA CC130 containing the mecA homologue mecALGA251 were reported from the UK and from Denmark so far from cattle and humans. Here we report on 11 MRSA CC130 among a sample of 12691 isolates of human origin collected from January 2006 until June 2011. MRSA CC130 grew insufficiently on chromogernic agar plates for detection of MRSA; the agglutination test for presence of PBP2a was negative. We designed primers for specific detection of mecALGA251 as well as for concomitant detection of both, mec LGA251 and mecA. As already described, the isolates exhibited spa-types t843, t1736, and t1773. The ccrA homologue indicated the presence SCCmec XI. When subjected to further characterization by means of a commercially available microarray the isolates were negative for sak chp, and scn, and as expected positive for hla, untruncated hlb, and hld. They furthermore contained edinB, aur, slpA, slpB, slpE. From genes coding for surface and cell wall associated products the ica-operon, cap8, clfA, clfF, ebpS, fnbA, fnbB, sdrC were detected but not cna. The isolates were negative for enterotoxin genes and tst, as well as for eta, and etb; agr-type was III.
Highlights
Nosocomial infections with methicillin resistant Staphylococcus aureus (MRSA) became an infection control problem worldwide during the past 20 years. They are mainly associated with hospital associated, clonal lineages (HA-MRSA) which have a pronounced capacity for spread in and among hospitals [1]
There are several reports on mastitis in cattle associated with LA-MRSA ST398
The 11 isolates MRSA ST130 investigated were only weakly able to grow on commercially available selective agar plates for MRSA
Summary
Nosocomial infections with methicillin resistant Staphylococcus aureus (MRSA) became an infection control problem worldwide during the past 20 years. They are mainly associated with hospital associated, clonal lineages (HA-MRSA) which have a pronounced capacity for spread in and among hospitals [1]. Earlier studies based on phenotypic characterization suggested that S. aureus from cattle and from humans are unrelated [7]. This was confirmed by molecular typing for clonal lineages ST97 and ST705 of bovine origin, which seem to be pandemic [8,9]. There are several reports on mastitis in cattle associated with LA-MRSA ST398
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