Rare endemic plants of the mountainous Crimea <i>Crepis purpurea </i>(Willd.) M. Bieb.) and <i>Scrophularia exilis</i> Popl. preservation under <i>in vitro</i> genebank conditions

Abstract

The article presents for the first time the results of a study of the preservation features of explants of 2 rare endemic species under the conditions of the in vitro genebank for 12 months. Microshoots of Crepis purpurea (Willd.) M. Bieb (Asteraceae) and Scrophularia exilis Popl. (Scrophulariaceae), cultured in vitro were included in the research. Microshoots’ segments 0,5-1,0 cm long were used as explants for preservation. The explants were placed on an agarized culture medium with a recipe of ¼ MS supplemented with growth inhibitors: 0,2 g/l CCC chlorocholinchloride (BASF, Germany) and 60 g / l sucrose (Panreac, Spain). As a control, a medium of ¼ MS was used, supplemented with 60 g / l sucrose without retardants. Culture vessels were placed in refrigerators with a light intensity of 1.25-3.75 mcM m -2 s -1 and a temperature of 4, 6, 8, 10 and 12,0°C. Plant material was evaluated after 6 and 12 months of cultivation using qualitative and quantitative characteristics of explants. It was found that the preservation of viability and reduction of growth kinetics of Crepis purpurea and Scrophularia exilis explants during 12 months of deposition in the in vitro genebank, along with low illumination intensity and the use of a nutrient medium with growth inhibitors, was facilitated by exposure to a temperature of 4,0-6,0°C. Conservated explants of the studied genotypes after transferring to standard cuture conditions the morphogenetic potential and the ability to regenerate microshoots and microrosettes were remained.