Rare Bacteria Can Be Used as Ecological Indicators of Grassland Degradation

Abstract

Grassland degradation is a major ecological problem at present, leading to changes in the grassland environment and the soil microbial community. Here, based on full-length 16S rRNA gene sequencing, we highlight the importance of small-scale environmental changes on the Qinghai-Tibet Plateau grassland for the composition and assembly processes of abundant and rare bacterial taxa. The results showed that grassland vegetation coverage affected the taxonomic and phylogenetic composition of rare bacterial taxa more than abundant bacterial taxa. The taxonomic composition and phylogenetic composition of rare bacterial taxa were also affected by soil nutrients. The relative contribution of deterministic processes (variable selection and homogeneous selection) to rare bacterial taxa was higher than that of the abundant bacterial taxa. The competitive potential within rare bacterial taxa was lower than that of the competitive potential between rare and non-rare bacterial taxa or within non-rare bacterial taxa. The assembly of rare bacterial taxa was more susceptible to environmental changes caused by grassland degradation than the abundant bacterial taxa. Furthermore, the distribution of rare bacterial taxa in the different degraded grassland soil was more local than that of abundant bacterial taxa. Thus, rare bacterial taxa could be considered an ecological indicator of grassland degradation. These findings help to improve our understanding of the composition and assembly mechanism of the bacterial communities in degraded grassland and provide a basis for the establishment of the grassland degradation management strategy.