Abstract
Carrot cells in suspension culture were incubated during the log-phase of the culture transfer cycle for different periods with one of the following precursors of nucleic acid synthesis: [32P]-orthophosphate, [5,6-3H]-uridine, and [2-14C]-uridine. Cells were gently broken by a short period of sonication, and the total RNA of the cells was extracted by a phenoldetergent method at pH 9.0. Subsequently, crude RNA was purified from contaminating substances like carbohydrates and nucleotides, and the pure RNA preparations were characterized by MAK-chromatography and constant velocity sedimentation in isokinetic sucrose gradients.
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