Abstract
Polydopamine (PDA) coating provides a promising approach for immobilization of biomolecules onto almost all kinds of solid substrates. However, the deposition kinetics of PDA coating as a function of temperature and reaction method is not well elucidated. Since dopamine self-polymerization usually takes a long time, therefore, rapid-formation of PDA film becomes imperative for surface modification of biomaterials and medical devices. In the present study, a practical method for preparation of rapidly-deposited PDA coating was developed using a uniquely designed device, and the kinetics of dopamine self-polymerization was investigated by QCM sensor system. It was found that high temperature and vigorous stirring could dramatically speed up the formation of PDA film on QCM chip surface. Surface characterization, BSA binding study, cell viability assay and antibacterial test demonstrates that the polydopamine coating after polymerization for 30 min by our approach exhibits similar properties to those of 24 h counterpart. The method has a great potential for rapid-deposition of polydopamine films to modify biomaterial surfaces.
Highlights
Inspired by the composition of adhesive proteins in mussels, Messersmith et al [1] (2007) employed the innate self-polymerization attribute of dopamine to form thin and surface-adherent polydopamine (PDA) film, which later on has been widely applied as a surface modification agent
The main aim of this study is to investigate the deposition kinetics of PDA film as a function of temperature and stirring, and to develop a method for rapidly-deposited PDA coating under standard environment (2 g?L21 dopamine in 10 mM Tris-HCl, pH = 8.5).In this investigation, the impact of temperature and reaction method on the deposition kinetics of PDA coating was evaluated by quartz crystal microbalance (QCM) sensor system, which is commonly used to monitor changes in mass at the quartz crystal sensing surface and capable of real-time in situ detection in solution [27]
The influence of temperature and stirring on the deposition kinetics of PDA film was investigated by the quartz crystal microbalance (QCM)
Summary
Inspired by the composition of adhesive proteins in mussels, Messersmith et al [1] (2007) employed the innate self-polymerization attribute of dopamine to form thin and surface-adherent polydopamine (PDA) film, which later on has been widely applied as a surface modification agent. To author’s knowledge, dopamine solution is usually polymerized for at least several hours prior to surface modification/deposition. It is too long from practical point of view and it is crucial to develop a rapid PDA film formation method. It is known that the process of dopamine-polymerization first involves oxidation of a catechol to a benzoquinone [13] and PDA film is a complex network with free catechol groups available for further chemical surface modification [14]. Recent research suggests that PDA is a supra-molecular aggregate of monomers rather than a covalent polymer [15]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
