Abstract
A fiber optic array (FOA) can be used as an alternative or a supplement to the lens in a microscope due to its large magnification, high coupling efficiency and extremely low distortion. Based on our previous research, this paper first demonstrated the resolution and field-of-view (FOV) of the microscope based on the FOA. To further validate the FOA microscope’s imaging capability, yeast activity and concentration were investigated by simple image processing. The results showed that the percentages of live and dead yeast cells correctly identified were 92.1% and 84.8%, except for the clusters, which agreed well with the manual counting methods. Then, the performances of the portable microscopes based on the FOA and lens were compared and the factors that affect the FOA microscope imaging performance were analyzed.
Highlights
To address the demand for diagnosing parasites in remote areas and telemedicine, portable microscopes based on lenses have achieved rapid deployment due to their simple principles and operation [1,2,3,4,5,6,7].due to the inherent characteristics of the objective lens, this microscope has limitations which include size, large optical distortions or aberrations and tedious focusing. the distortion can be partly overcome by an achromatic objective lens, the demands for light-weight and cheap portable microscopes have limited its use
The distortion can be partly overcome by an achromatic objective lens, the demands for light-weight and cheap portable microscopes have limited its use
As an alternative to the lens, a fiber optic array (FOA) can be used as an amplifier component due to its distortionless imaging, high magnification and high coupling efficiency, which provides a new idea for portable microscopes
Summary
As an alternative to the lens in the microscope, FOA can be used to magnify the image of the specimen that is placed on the small end of FOA, and project it to the CMOS sensor [15]. This solution reduces the device’s volume and improves the light transmission efficiency [16]. The related resolution and yeast cellcell test,test, while the the inset is the image captured by using the commercial digital microscope. The yeast cell detection and the image captured by using the commercial digital microscope.
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