Rapid typing of foot-and-mouth disease serotype Asia 1 by reverse transcription loop-mediated isothermal amplification

Hao-Tai Chen,Yong-Sheng Liu,Xiang-Tao Liu,Jie Zhang

doi:10.1186/1743-422x-8-489

Abstract

A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was rapidly used to detect serotype Asia 1 of foot-and-mouth disease virus (FMDV) within 45 min at 61°C. All FMDV serotype Asia 1 reference strains were positive by RT-LAMP, while other viruses such as FMDV serotypes O, C, A and classical swine fever virus, swine vesicular disease virus, porcine reproductive and respiratory syndrome virus and Japanese encephalitis virus remained negative. Furthermore, FMDV sreotype Asia 1 positive samples were able to detect by RT-LAMP assay. This RT-LAMP assay may be suitable particularly for diagnosis of FMDV serotype Asia 1 infection in field stations.

Highlights

A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was rapidly used to detect serotype Asia 1 of foot-and-mouth disease virus (FMDV) within 45 min at 61°C
Article Outline Foot-and-mouth disease virus (FMDV) is a member of the genus Aphthovirus of the family Picornaviridae, which is divided into seven serotypes with no cross-protection conferred among the serotypes [1]
RTLAMP assay was developed a diagnostic method for the typing of FMDV serotype Asia 1, and the sensitivity and specificity of the RT-LAMP assay were evaluated using the clincal samples

Summary

Introduction

A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was rapidly used to detect serotype Asia 1 of foot-and-mouth disease virus (FMDV) within 45 min at 61°C. Due to the aggressive nature of foot-and-mouth disease (FMD), outbreaks usually result in severe economic losses and impact on both national and international trade within the livestock and animal products [4,5,6]. * Correspondence: liuyongshengvip8@163.com; hnxiangtao@hotmail.com † Contributed State Key Laboratory of Veterinary Etiologic Biology, National Foot-andMouth Disease Reference Laboratory of China, Key laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, P.R. China (RT-PCR) [5,10,11,12,13,14] and real-time RT-PCR [6,15,16,17,18] were developed to complement primary diagnostic techniques for the FMDV infection.

Results

Conclusion