Abstract
Sedum telephium is a C 3 /CAM intermediate plant in which expression of CAM is caused by water deficit. The timing of the C 3 -CAM switch and its relationship with water status and phosphoenolpyruvate (PEP) carboxylase activity have been investigated. Water deficit was provided by application of polyethylene glycol (PEG) solutions so that roots were exposed to water potentials from 0 to −2.0 MPa below that of the nutrient solution. The response of the plants was measured during the first dark period after PEG addition and 7 d later. Malic acid accumulation was triggered during the first dark period at root water potentials of −0.3 MPa or less. This corresponded with very small decreases in leaf water potential and relative water content
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