Abstract
One titrimetric and two spectrophotometric methods have been described for the determination of ofloxacin (OFX) in bulk drug and in tablets, employing N-Bromosuccinimide as an analytical reagent. The proposed methods involve the addition of a known excess of NBS to OFX in acid medium, followed by determination of unreacted NBS. In titrimetry, the unreacted NBS is determined iodometrically, and in spectrophotometry, unreacted NBS is determined by reacting with a fixed amount of either indigo carmine (Method A) or metanil yellow (Method B). In all the methods, the amount of NBS reacted corresponds to the amount of OFX. Titrimetry allows the determination of 1-8 mg of OFX and the calculations are based on a 1:5 (OFX:NBS) reaction stoichiometry. In spectrophotometry, Beer's law is obeyed in the concentration ranges 0.5-5.0 µg/mL for method A and 0.3-3.0 µg/mL for method B. The molar absorptivities are calculated to be 5.53x10(4) and 9.24x10(4) L/mol/cm for method A and method B, respectively. The methods developed were applied to the assay of OFX in tablets, and results compared statistically with those of a reference method. The accuracy and reliability of the methods were further ascertained by performing recovery tests via the standard-addition method.
Highlights
This drug is official in the United States Pharmacopoeia (USP, 2004) and the British Pharmacopoeia (BP, 2003), which recommend non-aqueous titrimetry (USP, 2004; BP, 2003) and HPLC (USP, 2004) techniques for its assay in bulk and dosage forms
A close examination of the literature search presented in the introduction reveals that NBS has not yet been used for the spectrophotometric determination of OFX
The unreacted NBS is determined iodometrically, while in spectrophotometric methods, it is determined by reacting with a fixed amount of either indigo carmine and measured at 610 nm (Figure 2) or metanil yellow and measured at 530 nm (Figure 2)
Summary
A review of the literature revealed that various analytical methods for the determination of OFX in bulk and pharmaceutical dosage forms have been reported These include spectrofluorometry (Salem, 2005), atomic absorption spectrometry (Salem, 2005), capillary electrophoresis (Abdalla et al, 2008; Sun, Wu, 1999), HPLC (Arjekar et al, 1996; Carlucci et al, 1993; Kalta, Sharma, Chaturvedi, 2008; Sinde, Desai, Tendolkar, 1998), HPTLC (Srividya, Cardoza, Amin, 2003), chemiluminescence (Francis, Paul, Adcock, 2005), as well as electroanalytical (Tamer, 1990; Tuncel, Atkosar, 1992; Zhou, Pan, 1995) and microbiological methods (Silveria, Schapoval, 2002). The methods developed offer the advantages of simplicity, sensitivity, speed, accuracy and precision without the need for costly equipment/chemicals
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
