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Abstract
The toxic function of deoxynivalenol (DON) is the 12,13-epoxy-group because of its potential to inhibit protein biosynthesis. A possible way to detoxify DON is microbiological transformation of the epoxide to a diene. The aim of this work was to develop rapid analytical methods for monitoring of anaerobic and aerobic microbiological transformation experiments with DON. Thin-layer-chromatography (TLC) is beside high pressure liquid chromatography (HPLC) and gas chromatography GC known as a conventional method for the determination of trichothecene mycotoxins. We developed a TLC method with silica gel 60 plates for the determination of the aerobic transformation products of DON and a RP-TLC method for the determination of anaerobic metabolites. Without the need of any clean-up these methods give extremely rapid results at a very low cost.
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