Rapid spectrophotometric determination of nitrates and nitrites in marine aqueous culture media

Abstract

The spectrophotometric determination of nitrate in sea water broths for cultivation of, say, microalgae is complicated by the frequent presence of nitrite. Two methods - sulphamic/perchloric acid method (also known as Cawse method) and sul- phamic acid method - both claimed to be able to eliminate nitrite interference, were tested using a set of standards, but statist i- cal treatment of the results proved their limitations in nitrate quantification. An improved method, based on former published methods for quantification of nitrite and coupled determination of nitrate and nitrite, was designed and tested. This improved method was compared with the reference method (based on use of a cadmium column) using several standards and biological samples of two culture media for microalgae, in different phases of their growth curve. The results thus obtained have demon- strated that there is no statistically significant difference between them at the 5% level. The precision of the method was tes ted by repeating determinations with three sets of standard mixtures containing nitrate and nitrite. The method proposed has advan- tages over conventional methods in reduced time of analysis, as well as high precision and accuracy, so it may be a good alter- native for determination of nitrite and nitrate in marine aqueous media.