Abstract
A rapid, label-free assay using photonic crystal biosensors for the direct detection of intact viable rotaviruses is demonstrated. This paper describes the design and operation of the biosensor, surface functionalization with anti-rotavirus antibody, characterization of the detection sensitivity, and demonstration of specificity with respect to non-target virus particles. A 30-min assay of a partially processed water sample yielded a detection sensitivity of 36 virus focus forming units without the use of any external reagents. This sensitivity is comparable to commercial enzyme-linked immunosorbent assays. These results suggest photonic crystal biosensors may be useful for real-time monitoring of virus contamination of environmental water resources.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.