Abstract
Abstract An improved reversed-phase high performance liquid chromatographic technique for the rapid simultaneous analysis of patecnolamines is presented. The catecholamines are preconcentrated by employing a single preconcentration step, solid-liquid extraction, with active alumina. A C18 reversed-phase column is used and the catecholamines are detected at 280 nm. the retention time is 3.7 min for norepinophrine, 4.5 min for epinephrine and 6.2 min for dopamine using an eluting system of 0.165 M AcOH 1 pc in MeOH. The within-run precision CVs are in the order of 7 pc whereas the day-to-day precision CVs are in the order of 10 pc for concentrations near the upper normal limits. the application of the technique to a series of hypertensive subjects indicated, after comparison with three patients with than sixfold of upper normal limits in addition to fourfold or more increase in DA excretion seems to be indicative for the presence of malignant pheochfomocytoma. to assess the efficiency of such a discriminati...
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