Abstract
SummarySexing of human DNA in biological stains can be performed by amplifying a fragment of the X-Y homologous amelogenin gene. We analysed the fluorescent X and Y specific PCR-fragments by capillary gel electrophoresis (CGE) with laser induced fluorescent (LIF) detection. The CGE-procedure was optimised with respect to separation and analysis-time. We validated the CGE-LIF procedure by comparing the obtained XY-sextyping results with the typing results obtained by denaturing Polyacryl-amide gelelectrophoresis with automated detection of the alleles using an ABI 373 A automated sequencer. It was found that fluorescence-based PCR together with CGE and LIF detection provided a reliable sex-typing procedure. Besides, extremely fast run times (3 minutes) were possible using replaceable gelmedia and pressure injection.
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