Rapid serum agglutination, cultural isolation and PCR for detection of M. gallisepticum and M. synoviae infection in poultry

Abstract

Poultry mycoplasmosis, is an infectious disease of birds distributed worldwide causing serious economic loss to the poultry industry. Diagnosis of poultry mycoplasmosis is considered to difficult task due to different aspects of etiological agent from conventional bacteria. Therefore, the present study was aimed to detect mycoplasma infection in poultry using serological, cultural and molecular techniques. The specimens included were, sera samples and choanal swabs (150 each) collected from 150 birds housed in semi arid and costal area of Maharashtra (India). Mycoplasma gallisepticum (MG) was more prevalent than Mycoplasma synoviae (MS) in mycoplasmosis detected by rapid serum agglutination test (RSA), cultural isolation and direct PCR of samples. Nucleotide sequences of three representative MG isolates with NCBI accession nos. KY467400, KY467401 and KY467403 did not show any variations in their sequences analysed and matched with published strains of MG. RSA, cultural isolation and direct PCR yielded 93 (62%), 24 (16%) and 85 (56.67%) positive cases respectively. The sensitivity of PCR and RSA was 95.83% and 83.33% respectively. Thus, RSA and PCR were better than cultural isolation in diagnosis of poultry mycoplasmosis, therefore these methods can be used for screening the flocks for detection of mycoplasma infection depending on availability of specimens and facility.