Rapid serological typing of herpes simplex virus and titration of herpes simplex antibody by the use of mixed hemadsorption ? a mixed antiglobulin reaction applied to virus infected tissue cultures

Abstract

The mixed hemadsorption technique, which is essentially a mixed antiglobulin reaction, was used for a direct serological typing of freshly isolated strains of herpes simplex virus in partly degenerated tissue culture tubes. By this method isolation and typing can generally be carried out within 2 or 3 days. The same method was utilized also for the titration of antibody in human sera. Very high specific titers were obtained for post-infectious sera. The height of these titers was only moderately correlated to those obtained by complement fixation and neutralization tests.