Abstract
In a rotating tube with steady (laminar) flow (RSF) or with alternate flow (RAF) method for separation of fine particles, a simple and practical formula giving the relationship between the particle velocity (RSF velocity) along the tube length and parameter Aω0/V0 is derived, where A is the tube radius, ω0 is its angular velocity, and V0 is the sedimentation velocity of a particle. The chromatographic classification by these helical orbit sedimentation methods with laminar flow have been investigated with the formula in detail. In the flow methods the lowest size for biological and chemical samples with density ρ(≳1.05 g/cm3) is estimated as ∼10 μm when the tube with 2–4 mm i.d. and length L∼1 m is used.
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