Abstract
AbstractThe separation and resolution of the isopeptides Nϵ(γ‐glutamyl)‐L‐lysine and Nϵ(β‐aspartyl)‐L‐lysine formed in heated proteins has been successfully achieved. The method demands a well‐characterised ion‐exchange column and the use of pH 3.40 lithium citrate buffer (0.2 N Li+). Due to the variations in particle size and cross‐linkages in the ion‐exchange resin a computer‐assisted buffer gradient system has been developed. This system effects resolution of both isopeptides in 7 h. The use of leucyl‐glycine as an internal standard facilitates quantitative estimation of the isopeptides.
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