Abstract
A colorimetric method has been developed for the routine determination of estrogens in pharmaceutical preparations. The method is based on the formation of an azo dye from the condensation of diazotized 4-amino-6-chloro-m-benzene disulfonamide with estrogens which contain a phenolic hydroxyl group. The condensation product exhibits a red color with an absorption maximum at 500 mμ in alkaline solution. The most reproducible results are obtained when coupling is carried out at pH 5 buffered solution. The color formation obeys Beer's law over a range of 0.05–0.25 mg. of estrogens per ml. of sample solution. Optimum conditions for the color formation have been determined, and the application of this procedure to pharmaceutical products is given. A colorimetric method has been developed for the routine determination of estrogens in pharmaceutical preparations. The method is based on the formation of an azo dye from the condensation of diazotized 4-amino-6-chloro-m-benzene disulfonamide with estrogens which contain a phenolic hydroxyl group. The condensation product exhibits a red color with an absorption maximum at 500 mμ in alkaline solution. The most reproducible results are obtained when coupling is carried out at pH 5 buffered solution. The color formation obeys Beer's law over a range of 0.05–0.25 mg. of estrogens per ml. of sample solution. Optimum conditions for the color formation have been determined, and the application of this procedure to pharmaceutical products is given.
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