Abstract
A strategy for biocatalyst immobilization in flow directly from the crude cell extract is described.
Highlights
A strategy for biocatalyst immobilization in flow directly from the crude cell extract is described
We extend the HaloTagTM method to a continuous biocatalytic production process involving the alcohol dehydrogenase from Lactobacillus brevis (LbADH).[7]
In this study we demonstrate an easy and rapid preparation of a packed-bed flow reactor by immobilizing the fusion enzyme directly from the crude cell extracts from the respective recombinant E. coli production host immediately prior to conducting the biotransformation
Summary
A strategy for biocatalyst immobilization in flow directly from the crude cell extract is described. The efficiency and the stability of the immobilized enzyme were demonstrated during the asymmetric reduction of a range of ketones.
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