Abstract
BackgroundBecause many Miscanthus genotypes can be cultivated with relatively high productivity and carbohydrate content, Miscanthus has great potential as an energy crop that can support large scale biological production of biofuels.ResultsIn this study, batch hydrothermal pretreatment at 180°C for 35 min followed by enzymatic hydrolysis was shown to give the highest total sugar yields for Miscanthus x giganteus cv. Illinois planted in Illinois. High throughput pretreatment at 180°C for 35 min and 17.5 min followed by co-hydrolysis in a multi-well batch reactor identified two varieties out of 80 that had significantly higher sugar yields from pretreatment and enzymatic hydrolysis than others. The differences in performance were then related to compositions of the 80 varieties to provide insights into desirable traits for Miscanthus that enhance sugar yields.ConclusionsHigh throughput pretreatment and co-hydrolysis (HTPH) rapidly identified promising genotypes from a wide range of Miscanthus genotypes, including hybrids of Miscanthus sacchariflorus/M. sinensis and Miscanthus lutarioriparius, differentiating the more commercially promising species from the rest. The total glucan plus xylan content in Miscanthus appeared to influence both mass and theoretical yields, while lignin and ash contents did not have a predictable influence on performance.
Highlights
Because many Miscanthus genotypes can be cultivated with relatively high productivity and carbohydrate content, Miscanthus has great potential as an energy crop that can support large scale biological production of biofuels
The data reported for the 80 Miscanthus genotypes include averages, minimum and maximum contents of glucan, xylan, the total glucan plus xylan as carbohydrates, and lignin
The glucan or xylan yields without pretreatment did not show correlations to those glucan and xylan yields for the pretreatment at 17.5 min and 35 min (Additional file 3: Figure S1). These results suggested that sugar yields from direct enzymatic hydrolysis of raw lignocellulosic biomass without pretreatment were not suitable predictors of sugar yields from enzymatic hydrolysis of pretreated biomass
Summary
Because many Miscanthus genotypes can be cultivated with relatively high productivity and carbohydrate content, Miscanthus has great potential as an energy crop that can support large scale biological production of biofuels. A move from fossil to renewable fuels is vital if we hope to slow the impacts of carbon dioxide accumulation on global climate. Against this background, production of fuels from cellulosic biomass is one different locations in Europe, a growth model towards more robust yield predication on the basis of different climatic and soil condition was developed in Europe [9]. For biological processing to ethanol and other products, it is beneficial to identify genotypes that are most processed into sugars
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