Rapid screening of 2-[ [formula omitted]]-fluoro-2-deoxy-D-glucose infusions for volatile organic compound contaminants by solid phase microextraction with gas chromatography—selective ion monitoring mass spectrometry (SPME-GC-SIMMS)

Abstract

A method compatible with radioactive samples, capable of detecting trace volatile components in a sample volume of ca. 1 cm 3 of 2-[ 18 F ]-fluoro-2-deoxy-D-glucose solution is described. The approach, based on solid phase micro-extraction gas chromatography-mass spectrometry with a carboxen/polydimethylsiloxane based fibre, was optimised with respect to extraction time ( 10 min ), extraction temperature (60°C) and phase volume ratio (1). The analysis time, including extraction, was less than 20 min with linear responses for acetonitrile and ethanol over the ranges: 0.09– 80 μg cm −3 (22°C, acetonitrile) and 0.78– 79 μg cm −3 (22°C, ethanol). The detection limits were estimated to be ca. 0.78 μg cm −3 for ethanol and 0.09 μg cm −3 for acetonitrile. Stability studies indicated analyte losses of up to 75% over 24 h and analysis of aged 2- [ 18 F] FDG samples showed that levels of ethanol and acetonitrile were not less than 100 μg cm −3 , indicative of levels substantially greater than this in the original infusions given to human subjects.