Rapid Screening for FlavoneC-Glycosides in the Leaves of Different Species of Bamboo and Simultaneous Quantitation of Four Marker Compounds by HPLC-UV/DAD

Jin Wang,Hao Jiang,Feng Tang,Yong-De Yue

doi:10.1155/2012/205101

Abstract

A strategy for analyzing flavone C-glucosides in the leaves of different species of bamboo was developed. Firstly, the flavone C-glycosides were extracted from the bamboo leaves (51 species in 17 genera) with methanol and chromatographed on silica gel 60 plates in automatic developing chamber (ADC2), and a qualitative survey using simple derivatization steps with the NP reagent was carried out. The flavone C-glycosides were found in 40 of 51 species of bamboo examined. Secondly, an HPLC method with photodiode array and multiple wavelength detector was optimized and validated for the simultaneous determination of flavone C-glycosides, including isoorientin, isovitexin, orientin, and vitexin in the leaves of three species of bamboo and the flavone C-glycosides were confirmed by LC/MS. The optimized HPLC method proved to be linear in the concentration range tested (0.2–100 μg/mL, r 2 ≥ 0.9997), precise (RSD ≤ 1.56%), and accurate (88–106%). The concentration ranges of isoorientin, isovitexin, orientin, and vitexin in three bamboo leaves samples were 1.00–2.78, 0–0.31, 0–0.07, and 0.20–0.68 mg/g, respectively. The proposed method was validated to be simple and reliable and can be a tool for quality control of bamboo leaf extract or its commercial products.

Highlights

Bamboo leaves have been used in traditional Chinese medicine for treating fever and detoxification for over 1000 years
The main functional components of the extract from bamboo leaf are flavone C-glycosides including isoorientin, isovitexin, orientin, and vitexin (Figure 1), which have been used as marker compounds for the determination of commercial products of bamboo-leaf flavonoids
The standard compounds of isoorientin, orientin, isovitexin and vitexin were purchased from Shanghai Winherb Medical S & T Development Co., Ltd. (Shanghai, China)

Summary

Introduction

Bamboo leaves have been used in traditional Chinese medicine for treating fever and detoxification for over 1000 years. The main functional components of the extract from bamboo leaf are flavone C-glycosides including isoorientin, isovitexin, orientin, and vitexin (Figure 1), which have been used as marker compounds for the determination of commercial products of bamboo-leaf flavonoids. To investigate and determine these flavone C-glycosides in the leaves of different species of bamboo is needed. HPLC analysis for selected bamboo species in this study was not reported. The work aimed to develop a two-step procedure to quickly survey a large number of bamboo leave samples (51 species in 17 genera) for the presence of flavone Cglycosides and to optimize and validate an HPLC method. For the simultaneous quantitation of marker compounds in different bamboo leaves and its commercial products

Experimental

Results and Discussion

Conclusion