Abstract
BackgroundChlamydia trachomatis may cause multiple different urogenital tract disorders, but current non-culture assays for rapid screening of C. trachomatis typically use immunochromatography-based methods. We established another new rapid non-culture method for detection of C. trachomatis based on the measurement of α-mannosidase enzymatic activity in urogenital tract specimens.MethodTo evaluate the performance of this method, α-mannosidase activities of C. trachomatis serotype D strain 、 and 29 standard strains related to clinical urogenital pathogens were investigated. Furthermore, 553 urogenital tract specimens were used for clinical assays via cell culture method and ligase chain reaction method (LCR), adopting an expanded gold standard.ResultsOnly C. trachomatis was positive for α-mannosidase activity among different types of microbes tested in the research. When prostate fluid specimens, which have some interfering activity, were excluded, the sensitivity and specificity of the enzymatic method were 91.8% (78/85) and 98.3% (409/416), respectively. There were no significant differences (P > 0.05).ConclusionsThese results showed that α-mannosidase activity could be utilised as a screening marker of C. trachomatis infection.
Highlights
Chlamydia trachomatis may cause multiple different urogenital tract disorders, but current non-culture assays for rapid screening of C. trachomatis typically use immunochromatography-based methods
In our developed assay, only C. trachomatis samples tested was positive for α-D-mannosidase activity; but OD512 of both other organisms and cell cultures which were not inoculated with C. trachomatis used in the study was all below 0.100, which fell into the range of negative results negative with 0.150 (OD512) as the cutoff value, even if the reactions were allowed to proceed for 1 h at 37°C
Our results showed that clinical specimens such as urethral discharge, cervical secretions, and vaginal secretions did not interfere with the chromogenic detection of α-D-mannosidase activity to screen for C. trachomatis, prostate massage liquid produced more false positive results
Summary
Chlamydia trachomatis may cause multiple different urogenital tract disorders, but current non-culture assays for rapid screening of C. trachomatis typically use immunochromatography-based methods. We established another new rapid non-culture method for detection of C. trachomatis based on the measurement of α-mannosidase enzymatic activity in urogenital tract specimens. A large number of methods have been established for screening and diagnosis of C. trachomatis infection [11] Few of these assays meet the requirements of outpatient diagnosis, especially in terms of sensitivity, specificity, time, and simplicity of operation. Non-culture assays for C. trachomatis screening typically adopt immunochromatography-based methods. The purpose of the study was to establish a novel screening method for C. trachomatis infection without culture that would be rapid and convenient for use in outpatient clinics
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