Abstract
Qing-Hua-Yu-Re-Formula (QHYRF), a new herbal preparation, has been extensively used for treating diabetic cardiomyopathy. However, the chemical constituents of QHYRF remain uninvestigated. In the present study, rapid ultrahigh-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) was used to qualitatively analyze the components of QHYRF. Qualitative detection was performed on a Kromasil C18 column through the gradient elution mode, using acetonitrile-water containing 0.1% formic acid. Twenty-seven compounds were identified or tentatively characterized, including 12 phenolic acids, nine monoterpene glycosides, two flavonoids, three iridoids, and one unknown compound. Among these, six compounds were confirmed by comparing with standards. A high-performance liquid chromatography (HPLC) method was developed to simultaneously determine the following six active components in QHYRF: danshensu, paeoniflorin, acteoside, lithospermic acid, salvianolic acid B, and salvianolic acid C. These HPLC chromatograms were monitored at 254, 280, and 320 nm. The method was well validated with respect to specificity, linearity, limit of detection, limit of quantification, precision, stability, and recovery. The HPLC-UV method was successfully applied to 10 batches of QHYRF.
Highlights
An increasing number of people have suffered from diabetes in recent years, and cardiovascular complications secondary to diabetes have become the main cause of death in diabetic patients. e incidence of cardiovascular disease in patients with diabetes is 2-3 times higher than that of nondiabetic patients [1]
Traditional Chinese herbal formulation (TCMF) has been widely used in clinic due to its well-proven efficacy and few side effects. e Qing-Hua-YuRe-Formula (QHYRF) is a new herbal preparation for treating Diabetic cardiomyopathy (DCM), which was developed by Professor Wang Xu, according to the clinic experience of Chinese Medicine Master Professor Zhou Zhongying. e recipe of QHYRF comprises six herbal medicines: radix rehmanniae recen, Journal of Analytical Methods in Chemistry
Application to Di erent Batches of QHYRF. e established high-performance liquid chromatography (HPLC)-UV method was subsequently employed for the simultaneous quanti cation of danshensu, paeoni orin, acteoside, lithospermic acid, salvianolic acid B, and salvianolic acid C in QHYRF from 10 di erent batches
Summary
An increasing number of people have suffered from diabetes in recent years, and cardiovascular complications secondary to diabetes have become the main cause of death in diabetic patients. e incidence of cardiovascular disease in patients with diabetes is 2-3 times higher than that of nondiabetic patients [1]. At present, controlling blood sugar and improving heart failure are the main ways to overcome DCM. E Qing-Hua-YuRe-Formula (QHYRF) is a new herbal preparation for treating DCM, which was developed by Professor Wang Xu, according to the clinic experience of Chinese Medicine Master Professor Zhou Zhongying. Clinical studies have shown that QHYRF could effectively improve symptoms in patients with DCM, including blood sugar, blood rheology, and left ventricular structure and function. Us, there is an urgent need to develop an effective method for QHYRF quality control, in order to guarantee its pharmacological efficacy. A simple, reliable, and sensitive analytical method, the high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method, was used to determine the quantity of the six major active components (danshensu, paeoniflorin, acteoside, lithospermic acid, salvianolic acid B, and salvianolic acid C) of QHYRF. A simple, reliable, and sensitive analytical method, the high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method, was used to determine the quantity of the six major active components (danshensu, paeoniflorin, acteoside, lithospermic acid, salvianolic acid B, and salvianolic acid C) of QHYRF. e potential application of the present study supports the quality control of QHYRF and provides a theoretical research basis for the further research of QHYRF in clinic
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