Abstract
A rapid-scanning fluorimeter based on constant-energy synchronous scanning is described. It is used as a liquid chromatographic detector for polyaromatic hydrocarbons (PAHs). The computerized system scans at a rate of 200 nm s −1 and collects data in the forward and reverse directions. Data are processed in real time. The system is tested for 3-component and 9-component mixtures of PAHs. A synchronous fluorescence spectrum is obtained for each PAH being eluted in the chromatogram. The spectral peaks observed in the rapid scan correspond to the absorption/fluorescence transitions of the PAHs with an overall vibrational energy loss equal to the Δν value chosen. Limits of determination for the PAHs are in the pg to low ng range. The system provides a reduced signal/noise ratio compared to the variable-wavelength fluorescence system for most PAHs examined.
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