Rapid quantitative detection for multiple antibiotics in honey using a quantum dot microsphere immunochromatographic strip

Abstract

We design a honey antibiotic detection system (HADS), which provides honey antibiotic residue rapid on-site detecting solution relying on simple sample preparation tactics, lateral-flow immunochromatographic assay (LFIA)-based strip and handheld sensor reader. Relying on the quantum dot microsphere probes conjugated to the antibiotic monoclonal antibodies, the LFIA strip can simultaneously detect sulfonamides (SAs) and tetracyclines (TCs) in high sensitivity with extremely low detecting limits of 0.4 μg/kg and good specificity. Moreover, the fabricated LFIA strips can be well conserved in vacuum-sealed bags at 4 °C for at least 6 months. Combining with the handheld sensor reader, both SAs and TCs can be quantitatively and automatically measured in high accuracy. All the operations including sample preparation and detection can be finished within 20 min at room temperature on site without any extra devices or complicated operations. • We design honey antibiotic detection system (HADS) for antibiotic residue detection. • HADS can detect sulfonamides (SAs) and tetracyclines (TCs) in high sensitivity. • HADS supports rapid (20 min) and cost-effective (75 cents) antibiotic detection. • HADS is proved with high accuracy, good stability, selectivity and specificity. • HADS supports rapid on-site detection for honey antibiotic residues.