Abstract
Lentinan is a clinically approved immune modulator and its anticancer and immunomodulatory bioactivity is found to be dependent on its triple helical conformation. Therefore, the development of rapid and convenient method for determination of bioactive lentinan with triple helical conformation holds great promise for the quality control of lentinan healthy products. In this work, an aniline blue fluorescent method was optimized and established to accurately and rapidly detect bioactive lentinan. In the presence of lentinan, the fluorescence intensity of aniline blue with 404 nm excitation and 492 nm emission dramatically enhanced within 15 min in pH 10 glycine-NaOH buffer solution, which allowed the analysis of lentinan in a very simple and fast manner. The method allowed for the sensitive determination of lentinan in the range of 1 to 60 μg/mL with a detection limit of 0.25 μg/mL. Notably, the protocol exhibited excellent selectivity for the determination of triple helical lentinan over other saccharides. The method was successfully applied to the detection of bioactive lentinan in health tonic solution, which demonstrated the method had great potential for quality control of lentinan contained products.
Highlights
Lentinan is isolated from the fruiting body of Lentinus edodes (Shiitake) [1] [2] and is known as a bioactive polysaccharide due to its strong antitumor activity through the activation of human immune system [1] [3] [4] [5] [6]
The method was successfully applied to the detection of bioactive lentinan in health tonic solution, which demonstrated the method had great potential for quality control of lentinan contained products
Fluorescence spectra of aniline blue in the absence and presence of lentinan were measured to investigate the feasibility of aniline blue fluorescent method to detect lentinan
Summary
Lentinan is isolated from the fruiting body of Lentinus edodes (Shiitake) [1] [2] and is known as a bioactive polysaccharide due to its strong antitumor activity through the activation of human immune system [1] [3] [4] [5] [6]. Lentinan has been approved as an immune modulator for tumor treatment and clinically ap-. With greater emphasis on the prevention of diseases, consumption of health products containing lentinan has grown in popularity. Recent researches have demonstrated that the immunotherapeutic activity of lentinan is mainly dependent on the polysaccharide’s junior and senior structures [9] [10]. Owing to the inter and intra molecular hydrogen bonds, lentinan can form the helical structures especially triple helical conformation in aqueous solution [15], which plays critical role in the immunoregulatory function [16] [17] [18]. Developing a rapid and convenient method for quantification of triple helical lentinan is highly desired to evaluate the quality of lentinan containing healthy products
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
