Abstract
In order to identify the quality of crude and processed Corydalis Rhizoma decoction pieces, the research established a simple, fast, reliable, and validated near-infrared qualitative and quantitative model combined with chemometrics. 51 batches of crude and 40 batches of processed Corydalis Rhizoma from the Zhejiang and Jiangsu provinces of China were collected and analyzed. Crude and processed Corydalis Rhizoma samples were crushed to obtain NIR spectra. The content of seven alkaloids in crude and processed Corydalis Rhizoma was determined by high-performance liquid chromatography (HPLC). Pretreatment methods were screened such as normalization methods, offset filtering methods, and smoothing. Combined with partial least squares-discriminant analysis (PLS-DA) and partial least squares (PLS), the qualitative and quantitative models of crude and processed Corydalis Rhizoma were established, and the correlation coefficient (R2), root mean square error of calibration (RMSEC), and root mean square error of prediction (RMSEP) were used as evaluation indexes. Tetrahydropalmatine was used as an example for screening pretreatment methods; the results showed that MSC combined with the second derivative and no smoothing and the model with the wavelength range of 10000–5000 cm−1 had the best predictive ability and applied to all seven alkaloid components. Among them, the correlation coefficients were all higher than 0.99, and RMSEC and RMSEP were all less than 1%. The qualitative and quantitative model of the seven alkaloids in Corydalis Rhizoma can effectively identify the crude and processed Corydalis Rhizoma and determine the content of the seven alkaloids. By studying the NIR qualitative and quantitative models of crude and processed Corydalis Rhizoma, we can achieve rapid discrimination and quantitative prediction of crude and processed Corydalis Rhizoma. These methods can greatly improve the efficiency of traditional Chinese medicine analysis and provide a strong scientific basis for the quality identification and control of traditional Chinese medicine.
Highlights
IntroductionCorydalis Rhizoma (referred as CR), named as Yuan Hu in Chinese, is the dried rhizome of Corydalis yanhusuo W
Corydalis Rhizoma, named as Yuan Hu in Chinese, is the dried rhizome of Corydalis yanhusuo W
Samples and Reagents. 61 batches of crude CR and 50 batches of processed CR from the Zhejiang and Jiangsu provinces of China were collected and analyzed, and 10 batches from each class of the samples were selected as external validation samples. ese medicinal materials have been identified by Professor Ping-fan Lai (Zhejiang Chinese Medical University). ese samples were kept in the Research Center of Chinese Medicine Processing Technology of the Zhejiang University of Traditional Chinese Medicine
Summary
Corydalis Rhizoma (referred as CR), named as Yuan Hu in Chinese, is the dried rhizome of Corydalis yanhusuo W. Produced in the Hebei, Shandong, Jiangsu, and Zhejiang provinces of China, CR has the effects of activating blood, moving Qi, and relieving pain in traditional Chinese medicine (TCM). According to the theory of TCM processing, the toxicity of drugs is reduced and the effects of moving Qi and relieving pain are enhanced by stirfrying with vinegar. Modern research showed that the seven free alkaloid compounds such as protopine, palmatine chloride, berberine, dehydrocorydaline, tetrahydropalmatine, tetrahydroberberine, and corydaline [3,4,5,6,7,8,9] in CR are the main active and characteristic components, Journal of Analytical Methods in Chemistry which are often used to evaluate the quality of CR. Modern pharmacological studies have indicated that the alkaloid compounds in CR have sedative, analgesic, antitumor, and other pharmacological effects [10,11,12,13,14,15]
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