Abstract
We evaluated a qualitative, solid-phase two-site immunochromatographic assay (ThyroChek; Franklin Diagnostics, Cedar Knolls, NJ) for identifying serum samples that contain thyrotropin (TSH) in a concentration >5 mU/L. The test is intended for use as a screening method for primary hypothyroidism. Reagents were provided to us by the manufacturer. The test is performed by adding 4 drops of serum (∼0.1 mL) to the well of the cassette. The serum is adsorbed onto the nitrocellulose membrane, exposed to the mobile-phase antibody to …
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