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Abstract
Abstract We found three isoforms (a, b, and c) of cystine lyase in broccoli ( Brassica oleracea var. italica ) inflorescence tissues. Cystine lyase b, the most abundant isoform, was rapidly purified to homogeneity. The native enzyme had a M r of 160,000 and composed of four identical subunits with a M r of 40,000. Thiocysteine and pyruvate were confirmed as reaction products. The purified cystine lyase b utilized l -cystine and S -alkyl l -cysteine sulfoxide as substrates. Other properties of cystine lyase b were almost similar to those of the isoform a as reported previously. Cystine lyase a and b were localized in cytosolic and/or vacuole fraction. A high activity of cystine lyase was detected in some Allium species in addition to Cruciferae plants.
Published Version
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