Abstract
A fast and cheap strategy for an efficient extraction of proteins from formalin-fixed and paraffin-embedded (FFPE) tissues is illustrated in this chapter. The described method is based on the exposure of the tissue samples to high temperature in a SDS-containing-Laemmli buffer that allows a good formaldehyde cross-link reversal. The protocol herein enables efficient extraction of non-degraded proteins that are suitable for western blot and other proteomic analyses.
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