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Abstract
Enteric and diarrheal diseases are a major cause of childhood illness and death in countries with developing economies. Each year, more than half of a million children under the age of five die from these diseases. We have developed a portable, microfluidic platform capable of simultaneous, multiplexed detection of several of the bacterial pathogens that cause these diseases. This platform can perform fast, sensitive immunoassays directly from relevant, complex clinical matrices such as stool without extensive sample cleanup or preparation. Using only 1 µL of sample per assay, we demonstrate simultaneous multiplexed detection of four bacterial pathogens implicated in diarrheal and enteric diseases in less than 20 min.
Highlights
Enteric and diarrheal diseases are one of the most common causes of death in children under the age of five, second only to pneumonia [1,2,3,4]
Differential diagnostics is important for pursuing the correct course of action; in the case of enterohemorrhagic E. coli, administration of antibiotics is counter-indicated, as it would cause the cells to lyse, releasing large amounts of toxin into the body
We present here a portable centrifugal microfluidic system that uses a sedimentation-based immunoassay, reported in previous work by our group [30,31,32,33], to detect a panel of four pathogenic bacteria: E. coli, Listeria, Salmonella, and Shigella
Summary
Enteric and diarrheal diseases are one of the most common causes of death in children under the age of five, second only to pneumonia [1,2,3,4]. Diarrhea is a significant problem in the developing world due to the scarcity of safe drinking water and sanitation Studies of this problem point to a cycle of poor health and nutrition leading to susceptibility to infection by enteropathogens, leading to acute and prolonged diarrhea that exacerbates poor health and malnutrition [5]. This cycle must be interrupted by access to both well-established and proven treatments, such as oral rehydration therapy, and diagnostic tools capable of identifying causative pathogens and informing targeted treatments and preventative measures. Differential diagnostics is important for pursuing the correct course of action; in the case of enterohemorrhagic E. coli, administration of antibiotics is counter-indicated, as it would cause the cells to lyse, releasing large amounts of toxin into the body
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