Rapid Photoinduced Single Cell Detachment from Gold Nanoparticle-Embedded Collagen Gels with Low Denaturation Temperature.

Chie Kojima,Yusuke Nakajima,Misaki Nishio,Kenji Takatsuka,Takeshi Kawano,Akikazu Matsumoto

doi:10.3390/polym12010213

Abstract

Cell Separation is important in various biomedical fields. We have prepared gold nanoparticle (AuNP)-embedded collagen gels as a visible-light-responsive cell scaffold in which photoinduced single cell detachment occurs through local thermal denaturation of the collagen gel via the photothermal effect of AuNP. Physicochemical properties of collagen materials depend on the origin of the collagen and the presence of telopeptides. In this study, we prepared various AuNP-embedded collagen gels by using different collagen materials with and without the telopeptides to compare their thermal denaturation properties and photoinduced single cell detachment behaviors. Cellmatrix type I-C without telopeptides exhibited a lower denaturation temperature than Cellmatrix type I-A and Atelocell IAC, as examined by Fourier transform infrared (FTIR) spectroscopy, rheological analysis, and sol–gel transition observation. Three-dimensional (3D) laser microscopic imaging revealed that collagen fibers shrank in Cellmatrix type I-A upon heating, but collagen fibers disappeared in Cellmatrix type I-C upon heating. Cells cultured on the Cellmatrix type I-C-based AuNP-embedded collagen gel detached with shorter photoirradiation than on the Cellmatrix type I-A-based AuNP-embedded collagen gel, suggesting that collagen gels without telopeptides are suitable for a photoinduced single cell detachment system.

Highlights

Cell separation is crucial to various biomedical applications, such as cell engineering and regenerative medicine [1,2,3]
We examined the thermal denaturation behaviors of various AuNP-embedded collagen gels with Fourier transform infrared (FTIR) spectroscopy, rheological analysis, sol–gel observations, and 3D microscopic imaging
The Cellmatrix type I-C-based collagen gel without telopeptides denatured at a lower temperature than those with telopeptides (Cellmatrix type I-A and Atelocell IAC), regardless of the presence of AuNPs

Summary

Introduction

Cell separation is crucial to various biomedical applications, such as cell engineering and regenerative medicine [1,2,3]. Because fluorescence-activated cell sorting (FACS) cannot be applied in spatiotemporal cell separations, image-guided cell sorting systems have been developed [4,5]. We have constructed a visible-light-induced single cell detachment system from gold nanoparticle (AuNP)-embedded collagen gels by using a microscope equipped with a laser irradiation system [6,7]. Collagen has the thermal denaturation properties and AuNPs have the photothermogenic property due to localized surface plasmon resonance [8,9]. The AuNP-embedded collagen gel can work as a visible-light-responsive cell scaffold.

Results

Discussion

Conclusion