Abstract

A simple, sensitive, rapid and inexpensive silica gel-impregnated glass fiber paper chromatographic method has been developed for the estimation of lecithin/sphingomyelin (L/S) ratio in amniotic fluid, using malachite green as staining reagent. Respiratory distress syndrome was not found in infants with high L/S ratios in normal and drug ‘methadone’ abuse pregnancies.

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