Rapid nucleus-scale reorganization of chromatin in neurons enables transcriptional adaptation for memory consolidation.

Manuel Peter,Thomas R Burkard,Anne Sinning,Heiko J Luhmann,Simon Rumpel,Florian Grössl,Dominic Kargl,Klaus Kraitsy,Dominik F Aschauer,Renata Rose,Wulf Haubensak

doi:10.1371/journal.pone.0244038

Abstract

The interphase nucleus is functionally organized in active and repressed territories defining the transcriptional status of the cell. However, it remains poorly understood how the nuclear architecture of neurons adapts in response to behaviorally relevant stimuli that trigger fast alterations in gene expression patterns. Imaging of fluorescently tagged nucleosomes revealed that pharmacological manipulation of neuronal activity in vitro and auditory cued fear conditioning in vivo induce nucleus-scale restructuring of chromatin within minutes. Furthermore, the acquisition of auditory fear memory is impaired after infusion of a drug into auditory cortex which blocks chromatin reorganization in vitro. We propose that active chromatin movements at the nucleus scale act together with local gene-specific modifications to enable transcriptional adaptations at fast time scales. Introducing a transgenic mouse line for photolabeling of histones, we extend the realm of systems available for imaging of chromatin dynamics to living animals.

Highlights

Interphase chromatin is organized in a highly ordered spatial structure with individual chromosomes occupying discrete chromosome territories within the nucleus [1,2,3,4]
To investigate and visualize nucleus-scale chromatin dynamics in neurons upon induction of neuronal activity, we followed a widely used strategy that preserves endogenous nucleosome organization [46]: We transduced cultured primary cortical neurons [44] with a recombinant adeno-associated virus encoding for the core nucleosome component histone H2B fused to the fluorescent marker mCherry (H2B::mCherry) (Fig 1A), and imaged labeled chromatin with a confocal microscope at a ten minutes interval
We tested if the observed changes in chromatin condensation are dependent on actomyosin activity and included a third condition in which we applied the pharmacological cocktail together with 20mM 2,3-butanedione monoxime (BDM)

Summary

Introduction

Interphase chromatin is organized in a highly ordered spatial structure with individual chromosomes occupying discrete chromosome territories within the nucleus [1,2,3,4]. This threedimensional nuclear architecture is believed to reflect the transcriptional state of the cell as the positioning of individual genes relative to transcriptional hotspots or the nuclear lamina is associated with their level of expression [5,6,7,8,9,10,11]. Most of this work relied on the experimental accessibility of cultured cells.

Methods

Results

Conclusion