Rapid Nanotoxicity Screening Approach of AgNPs Using Negative-charged Vesicle

Abstract

PP-29-166 Background/Aims: Silver nanoparticle (AgNP), one of commercialized nanomaterials, has received considerable attention in recent years, due to the potential toxicity to the environment and human. To investigate their potential nanotoxicity, OECD members are co-worked in fields of study; material characterization, environmental fate, risk assessment, environmental and mammalian toxicology, and material safety. Especially, in vivo and in vitro cytotoxicity results are increasingly being published. Methods: However, cytotoxicity studies of nanoparticles included different testing conditions, such as particle concentration, exposure times, and cell-lines. Therefore, it is difficult to determine rapidly whether the cytotoxicity reported is physiologically relevant. Herein, we proposed alternative rapid screening methodology of AgNPs' nanotoxicity, using biomimetic cell-membrane (negative phospholipid vesicle). Results: When the naked and coated (citrate) AgNP are inserted into the vesicle immobilized gold-chip of SPR instrument, we can in situ detect the cell-membrane penetration of AgNPs, which was confirmed by SPR's angle shift and TEM analysis. Conclusion: This different feature was due to the surface charge interaction between negative charge of vesicle and positive charge of naked AgNP, while citrate-coated AgNP has negative charge (COO−).