Abstract
Abstract Shoots tips from in vitro cultures of Heuchera sanguinea Engelm. ‘Rosamundi’ were cultured on a modified woody plant medium containing one of 3 cytokinins (BA, 2iP, kinetin) or cytokinin plus NAA. BA and kinetin were the most effective stimulators of axillary shoot growth. Maximum shoot production (≥1 mm) occurred with 2, 4, 8, and 16 μm BA, or 16, 32, and 64 μm kinetin. The addition of NAA had no effect or reduced the number of shoots produced. Because of the rosette growth pattern, rooting of the resultant shoots was best accomplished by placing whole cultures in a soilless medium under high humidity and later dividing the rooted shoot masses. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); N-(3-methyl-2-butenyl)-1H-purin-6-amine (2iP); N-(2-furanylmethyl)-1H-purin-6-amine (kinetin); and 1-naphthaleneacetic acid (NAA).
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