Rapid Multiplex PCR for Species Authentication: Current Prospects and Challenges

Abstract

Multiplex PCR is a powerful technique that allows simultaneous amplification of multiple DNA targets in a single reaction. It has been widely applied for species authentication in various food matrices, such as meat, fish, dairy, and honey products. Species authentication is important for ensuring food safety, quality, traceability, and compliance with religious, ethical, and environmental standards. However, multiplex PCR also faces some challenges and limitations, such as primer design, optimization, validation, interference, and interpretation of results. This review summarizes the current state of the art of multiplex PCR for species authentication, highlighting its advantages, applications, challenges, and future directions. It also provides some practical guidelines and recommendations for designing and performing multiplex PCR assays for reliable and accurate species identification.