Abstract
: The high demand of Oncidium orchids leads us to find out efficient methods of propagating them. However, the propagation rate of traditional methods are low in nature and a hybrid seed is not genetically stable. Thus, plant cell biotechnology is examined as the most effective way to resolve the barrier of elite clone production. Shoot clusters were cultured on MS medium supplemented with 2,4-D (1 mg/l) for callus induction (76.19%) before induced callus was favoured for PLBs regeneration (98 PLBs/callus cluster) on MS medium supplemented with NAA (0.75 mg/l); the combination of BA (0.5 mg/l) and NAA (0.5 mg/l) was favoured for PLBs regeneration (28.18 PLBs/shoot cluster) from shoots cultivation. The PLBs (79.21 PLBs/PLB cluster) were then proliferated on MS medium supplemented with NAA (1 mg/l) and BA (1 mg/l) for shoot regeneration (12.42 shoots/PLBs cluster). Multiple-shoots were divided to 3-4 shoots/cluster for micropropagation on the MS medium supplemented with the combination of BA (0.25 mg/l) and NAA (0.25 mg/l) to reach 11.66 shoots/cluster. Shoots were finally separated to single-shoot for rooting on the MS medium supplemented with NAA (0.75 mg/l). A scheme for Oncidium micropropagation using PLBs culture techniques was set up.
Highlights
Orchidaceae orchids include many of the flowers spices used in the cut flower industry
The culture of the shoot cluster on MS medium supplemented with 1 mg/l 2,4-D gave the highest incidence of formed callus with 76.19%
The protocorm like bodies (PLBs) culture from callus on medium supplemented with 0.75 mg/l NAA gave PLB as much as 98 PLB/callus
Summary
Orchidaceae orchids include many of the flowers spices used in the cut flower industry. Vu Nu (Oncidium) orchid is a fragrance that is popular in the world market in the form of flower pots and cut branches (Arditti, 2008). The technique of tissue culture has been successfully applied in the conservation and development of rare orchids, cut flowers, condiment and medicinal plants (Arditti, 2008). Vu Nu orchid culture has yielded the groundwork for the construction of micropropagation technology, such as the shoot tip, root tip, inflorescence culture of O. Sweet Sugar (Chen and Chang, 2000b) culture of young leaf producing direct and callus-formed PLBs (Chen and Chang, 2000a), proliferation of PLBs of O. Sweet Sugar (Hong et al, 2008)
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