Abstract
A hybrid between Salix Fragilis (female) x Salix Lispoclados (male) was established by ovary culture. The hybrid seedlings were grown on Schenk and Hildebrandt (SH) semi-solid medium containing 0.2 mg/L 6-benzyladenine (BA). Shoot multiplication from nodal cuttings of in vitro hybrid seedlings was achieved on semi-solid Woody Plant (WP) medium with 0.2 mg/L BA. The shoot proliferation could be enhanced to 5-fold upon transfer of shoots to liquid WP medium supplemented with 0.2 mg/L BA and incubation on a rotary shaker. Shoots became hyperhydric and developed smaller and less expanded leaves when they remained in liquid medium. Shoot vigor was restored upon transfer to semi-solid medium. Best rooting response of in vitro shoots was achieved on SH medium without hormone. The hybrid plantlets were successfully grown in the field.
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