Abstract
A procedure for the determination of total phenolic acid content in rapeseed/ canola was developed by combining and modifying two currently used methods. It consists of refluxing the sample with acidic acetone, alkaline hydrolysis of esterified phenolic acids followed by acidification, extraction with ethyl acetate/ ethyl ether, and colormetric measurement using Folin-Denis reagent. The method was tested with canola and casein samples spiked with sinapic acid or sinapine bisulphate standards. Recoveries ranging from 95 to 106% were obtained, and the detection limit was determined to be less than 5 mg sinapic acid equivalents per 100 g sample.
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