Abstract
A simple and rapid method is described for the estimation of some organophosphorus insecticides in the serum of occupationally exposed persons. The compounds are extracted with a mixture of acetone and diethyl ether (1:1 v/v) in acidic medium and the extraction residue is analyzed by gas chromatography with nitrogen phosphorus detection method. Linearity was acceptable over concentrations from 0.25 to 4.0 μg/mL. The method percentile recovery for the six different organophosphorus insecticides was 86.3% for phorate, 78.3% for dimethoate, 82.3% for malathion, 79.4% for chlorpyrifos, 80.2% for diazinon, and 68.5% for ethion at the μg/mL level. Serum samples of nine workers who had been occupationally exposed to malathion in an insecticide manufacturing factory, were analyzed and malathion was found at low levels in all the samples.
Highlights
A simple and rapid method is described for the estimation of some organophosphorus insecticides in the serum of occupationally exposed persons
In order Pravara Institute of to address these issues, a rapid, Medical Sciences had been occupationally exposed to malathion in an accurate, and sensitive method has (Deemed University), insecticide manufacturing factory, were analyzed and been developed for this application and Loni – 413736, malathion was found at low levels in all the samples
Extraction and Preparation of the Sample: One milliliter of serum was taken in a 10 mL glass stoppered tube to which was added 4 mL of a mixture of acetone and diethyl ether (1:1 v/v)
Summary
A simple and rapid method is described for the estimation of some organophosphorus insecticides in the serum of occupationally exposed persons. Standard Solutions Twenty millilitres of standard stock solutions of phorate, dimethoate, malathion, chlorpyriphos, diazinon, and ethion were prepared at a concentration of 1 mg/mL in acetone (HPLC Grade). Working solutions were prepared in ν-hexane by appropriate dilution of the standard stock solutions to obtain the desired concentrations of levels in blood, e.g., gas chromatography without sample extraction,[3,4] gas chromatography with sample extraction these organophosphate compounds in the range of 0.25 to 4.0 μg/mL.
Sign-in/Register to view highlights & summary 
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Indian Journal of Occupational and Environmental Medicine
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
