Abstract
An isocratic liquid chromatographic method has been developed for rapid measurements of retinol, retinal, all-trans-retinoic acid and 13-cis-retinoic acid. Using 85% methanol and 15% 0.01 M sodium acetate buffer, pH 5.2, as the mobile phase and at a flow rate of 1.5 mL/min, retinol, retinal, all-trans-retinoic acid and 13-cis-retinoic acid were eluted from a 5 μm C18 reversed-phase column (4.6 mm I.D., 15 cm) in less than 14 min. Retinyl acetate, when desired, was eluted in 25.5 min under the above conditions. Each compound was detected at the most sensitive wavelength by coupling fluorescence and UV detection.
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